在SDS - PAGE中,配膠時(shí)需加入TEMED和過(guò)硫酸銨,它的作用是什么?但將凝膠液加入在制膠器后需在其上慢慢加入一層乙醇或異丙醇或水
In SDS - page, TEMED and ammonium persulfate need to be added when preparing glue. What is its function? However, after adding the gel solution to the glue maker, slowly add a layer of ethanol, isopropanol or water onto it
聚丙烯酰胺(PAGE)是由丙烯酰胺聚合形成的高分子 這個(gè)聚合反應(yīng)是自由基聚合,需要有引發(fā)劑產(chǎn)生自由基將反應(yīng)引發(fā) 過(guò)硫酸銨就是引發(fā)劑,而TEMED可以催化過(guò)硫酸銨產(chǎn)生自由基,從而加速丙烯酰胺凝膠的聚合:
Polyacrylamide (page) is a polymer formed by polymerization of acrylamide. The polymerization reaction is free radical polymerization, which requires an initiator to generate free radicals. The initiator is ammonium persulfate. TEMED can catalyze ammonium persulfate to generate free radicals, thus accelerating the polymerization of acrylamide gel:
過(guò)硫酸鈉是一種無(wú)機(jī)化合物,化學(xué)式為Na2S2O8。它是過(guò)二硫酸的鈉鹽,是一種強(qiáng)氧化劑。它幾乎不潮解,可以長(zhǎng)時(shí)間儲(chǔ)存。過(guò)硫酸鈉可以通過(guò)硫酸氫鈉的電解氧化制得:2 NaHSO4 → Na2S2O8 + H2氧化在鉑陽(yáng)極發(fā)生。過(guò)硫酸鈉的標(biāo)準(zhǔn)氧化還原電位為2.1 V,高于過(guò)氧化氫(1.8 V),低于臭氧(2.2 V)。原位生成的硫酸根自由基(sulfate radical)的標(biāo)準(zhǔn)電極電勢(shì)為2.7 V。
Sodium persulfate is an inorganic compound with the chemical formula Na2S2O8. It is the sodium salt of persulfate and a strong oxidant. It hardly deliquesces and can be stored for a long time. Sodium persulfate can be prepared by electrolytic oxidation of sodium bisulfate: 2 NaHSO4 → Na2S2O8 + H2 oxidation occurs at platinum anode. The standard redox potential of sodium persulfate is 2.1 V, higher than hydrogen peroxide (1.8 V) and lower than ozone (2.2 V). The standard electrode potential of the sulfate radical generated in situ is 2.7 v.
過(guò)硫酸銨、過(guò)硫酸鈉、過(guò)硫酸鉀都是過(guò)氧化物,我知道的一個(gè)用途,是用作合成以水為溶劑/介質(zhì)的部分高聚物(塑料、樹(shù)脂)的引發(fā)劑。
Ammonium persulfate, sodium persulfate and potassium persulfate are peroxides. One use I know is to be used as initiators for the synthesis of some polymers (plastics and resins) with water as solvent / medium.
引發(fā)劑的用量是非常少的,通常只有樹(shù)脂量的1.5%左右。這些東西是分級(jí)出售的、根據(jù)純度來(lái)分的,工業(yè)級(jí)的、化學(xué)級(jí)的、分析純,是不一樣的,具體出廠不清楚。
The amount of initiator is very small, usually only about 1.5% of the amount of resin. These things are sold at different levels according to purity, industrial grade, chemical grade and analytical grade. The prices are different, and the specific ex factory prices are not clear.
過(guò)硫酸銨、過(guò)硫酸鈉、過(guò)硫酸鉀都是過(guò)氧化物,我知道的一個(gè)用途,是用作合成以水為溶劑/介質(zhì)的部分高聚物(塑料、樹(shù)脂)的引發(fā)劑。引發(fā)劑的用量是非常少的,通常只有樹(shù)脂量的1.5%左右。
Ammonium persulfate, sodium persulfate and potassium persulfate are peroxides. One use I know is to be used as initiators for the synthesis of some polymers (plastics and resins) with water as solvent / medium. The amount of initiator is very small, usually only about 1.5% of the amount of resin.
這些東西是分級(jí)出售的、根據(jù)純度來(lái)分的,工業(yè)級(jí)的、化學(xué)級(jí)的、分析純,是不一樣的,具體出廠不清楚。
These things are sold at different levels according to purity, industrial grade, chemical grade and analytical grade. The prices are different, and the specific ex factory prices are not clear.
儲(chǔ)存:過(guò)硫酸銨,但由于能釋放氧而有助燃作用,儲(chǔ)存時(shí),必須存放在干燥、密閉的容器中,避免陽(yáng)光直射、勿近熱源。不可同有機(jī)物和鐵銹、少量金屬等還原性物質(zhì)接觸,以防引起過(guò)硫酸銨的分解、爆炸。
Storage: ammonium persulfate, which can release oxygen and has combustion supporting effect, must be stored in a dry and closed container to avoid direct sunlight and close to the heat source. Do not contact with organic matter, rust, a small amount of metal and other reducing substances to prevent the decomposition and explosion of ammonium persulfate.
由于潮濕的過(guò)硫酸銨粉末及其水溶液有漂白和輕微的腐蝕作用,因此使用過(guò)程中因避免眼睛、皮膚和衣物直接與其接觸。
Because the wet ammonium persulfate powder and its aqueous solution have bleaching and slight corrosion, it is necessary to avoid direct contact with eyes, skin and clothes during use.
用途:
Purpose:
聚合:乳膠或丙烯酸單體聚合液、醋酸乙燃、氯乙烯等產(chǎn)品的引發(fā)劑,同時(shí)也是苯乙烯丙烯腈、丁二烯等膠體發(fā)生共聚作用的引發(fā)劑。
Polymerization: it is the initiator of latex or acrylic monomer polymerization solution, ethyl acetate, vinyl chloride and other products. It is also the initiator of copolymerization of styrene, acrylonitrile, butadiene and other colloids.
金屬處理:金屬表面的處理(例如在半導(dǎo)體制造業(yè):印刷電路的清潔和蝕刻)。銅和鋁表面的活化。
Metal treatment: treatment of metal surfaces (e.g. in semiconductor manufacturing: cleaning and etching of printed circuits). Activation of copper and aluminum surfaces.
化妝品:漂白配方的主要成分。
Cosmetics: the main component of bleaching formula.
紡織品:脫漿劑和漂白劑——特別為低溫漂白。
Textiles: desizing agent and bleaching agent - especially low temperature bleaching.
其他:化學(xué)合成:水處理(凈化);劑;廢氣處理,有害物質(zhì)氧化降解(例如汞);紙張(淀粉改性,為低溫漂白特殊再制漿)。
Others: chemical synthesis: water treatment (purification); disinfectant; Waste gas treatment, oxidative degradation of hazardous substances (e.g. mercury); Paper (starch modified, special re pulping for low-temperature bleaching).
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