在沒有溶解之前,廠家生產出來的EDTA二鈉大多數為粉末狀物質,在使用前需要將其配置成溶液后加入試管中。所以下面具體給大家介紹一下EDTA二鈉溶液的配置及注意的一些問題。
Before dissolution, most of the EDTA disodium produced by the manufacturer are powder substances, which need to be prepared into solution before use and then added into the test tube. So let's introduce the configuration of EDTA disodium solution and some problems to be noticed.
一、配置步驟
1、 Configuration steps
將EDTA二鈉粉末配置成溶液,其步驟大致如下:
To prepare EDTA disodium powder into solution, the steps are as follows:
1、準備適量的EDTA二鈉粉末,蒸餾水、氫氧化鈉溶液。
1. Prepare an appropriate amount of EDTA disodium powder, distilled water and sodium hydroxide solution.
2、將準備好的EDTA二鈉粉末中加入一定量的蒸餾水,使其溶解,然后用攪拌棒快速攪拌。攪拌過程中加入氫氧化鈉溶液,直將PH值調整到需要的范數值,帶EDTA二鈉溶解后,再次加入蒸餾水,將溶液定容所需要的位置。
2. Add a certain amount of distilled water to the prepared EDTA disodium powder to dissolve it, and then quickly stir it with a stirring rod. During the mixing process, add sodium hydroxide solution until the PH value is adjusted to the required value. After the solution is dissolved with disodium EDTA, add distilled water again, and bring the solution to the required volume.
3、然后用過濾器將溶液一次過濾,即可得到溶液,再將溶液裝到容器里,放置于高溫下使用,配置好的溶液在4度的溫度下可穩(wěn)定保存1年左右。
3. Then use a filter to filter the solution once to get the solution. Then put the solution into a container and put it under high temperature for sterilization. The prepared solution can be stably stored for about 1 year at 4 ℃.
二、配置注意問題
2、 Configuration considerations
為了不影響EDTA二鈉標準溶液的準確度,我們在配置的過程中要注意以下幾點:
In order not to affect the accuracy of EDTA disodium standard solution, we should pay attention to the following points in the process of configuration:
1、稱取EDTA二鈉粉末時,需注意準確控制用量,避免在配置過程中出現(xiàn)誤差性。在配置期間所用的溶液必須為蒸餾水配置,不含任何成分雜質,且使用的容器應用純凈水清洗3次以上。
1. When weighing EDTA disodium powder, attention should be paid to accurately control the dosage to avoid errors in the configuration process. The solution used during the preparation must be distilled water, free of any constituent impurities, and the container used should be washed with purified water for more than 3 times.
2、配置完成的溶液不能放在普通玻璃瓶中,因為瓶中的鈣離子可能會和EDTA產生反應,會影響實驗結果,盡量使用聚乙烯瓶或者硬質玻璃瓶。
2. The prepared solution cannot be placed in ordinary glass bottles because the calcium ions in the bottles may react with EDTA, which will affect the experimental results. Try to use polyethylene bottles or hard glass bottles.
3. The produced EDTA disodium powder is easy to transport and store. If it is prepared into a solution, it should be prepared and used immediately. Do not place the prepared solution for a long time, which will lead to deterioration and abnormal results. Come to our website for more relevant content http://www.hkjzshop.com Consult!